AAV Based Stable Cell Line Generation Services

Stable cell lines are generated by experienced scientists working on AAV and cell line development services for decades. Our proprietary AAV system is highly efficient in stable cell line development with any gene and any host cell, including mammalian primary cell. More copy number of the integrated gene via AAV-based method than that via DNA transfection method results in significantly improved efficiency of changing protein expression level.

Stable cell line generation using AAV:

AAV transduction into host cells and screening for cell lines with permanent integration of DNAs into cellular genome results in higher efficient protein overproduction or gene silencing. Our fast and predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity. The process includes:

- Molecular construction of AAV vectors with inserts from gene synthesis or templates provided by clients;
- AAV packaging and production;
- AAV transduction of host cells;
- Selection of cell pool or screening for stable single clones;
- Validation of stable pool or single clones by qPCR or specific methods such as FACS; (Note: The standard method for validation is qPCR. Additional fee will be charged for Western blot or cell-based assay of your interest.)
- Batch culture and cell banking.

Service of clonal stable cell line generation using AAV includes:

a. Single cell cloning;
b. Detailed clone characterization (viability and cell growth);
c. Backup vial storage for 6 months.

Deliverable:
a. 2 vials of the selected clone (5 million cells per vial);
b. Stable cell line development study report.

Fast turnaround: 12-14 weeks.

Service of cell pool generation using AAV includes:

Deliverable:
a. 2 vials of the final pool (5 million cells per vial);
b. Cell pool generation study report.

Fast turnaround: 8-10 weeks.

Celvab also provides the following viral-based cell line generation services:

Knock-out and Knock-in Cell Line Generation with CRISPR Genome Editing: development of stable cell lines for precise genomic modification.
Chimeric Antigen Receptor T-Cell Engineering: development of T-cells expressing chimeric antigen receptors.
Lentivirus Based Stable Cell Line Generation: development of stable cell lines using lentivirus alone or with AAV.