Endotoxin-free Plasmid DNA Production Service

BS047: Endotoxin-free DNA Production Service

Endotoxins are frequent contaminants in plasmid DNA and protein prepared from bacteria, and must be removed from the DNA or protein to avoid unwanted inflammatory responses prior to in vivo applications. Very low levels of LPS can cause coagulation of the limulus lysate due to a powerful amplification through an enzymatic cascade. The presence of endotoxin or lipopolysaccharide (LPS) can be sentitively detected by the Limulus Amebocyte Lysate assay, utilizing blood from the Horseshoe crab. The test was accepted by the United States Food and Drug Administration (FDA) in 1983.

Celvab provides endotoxin-free DNA production at 0.2 mg, 1 mg, and higher scale. The endotoxin levels in delivered samples are below 0.1 EU per microgram of DNA, consistent with industry standards (Qiagen and Sigma endotoxin-free products). Our endotoxin-free DNA products have been used in various in vivo and cell culture experiments, and have consistently shown excellent rates for transfection and expression. Final products are verified with either restriction enzyme digest or sequencing analysis, and are delivered with Certificate of Analysis.

Fast turnaround: 5 business days.

Related service:

Endotoxin Measurement Service